Synthetic and complex media for the rapid detection of fluorescence of phytopathogenic pseudomonads: effect of the carbon source.

Fluorescence is of diagnostic value for differentiating among species of aerobic pseudomonads (R. Y. Stanier, N. J. Palleroni, and M. Doudoroff, J. Gen. Microbiol. 43:159, 1966). The standard medium for detecting fluorescence is Medium B (E. 0. King, M. K. Ward, and D. E. Raney, J. Lab. Clin. Med. 44:301, 1954), which supports amino acids (J. De Ley, Ann. Rev. Microbiol. 18:17, 1964), and peptones (E. 0. King et al., J. Lab. Clin. Med. 44:301, 1954) affect fluorescence. The effect of carbon sources had not been shown. Although glycerol, glucose, or maltose can be used interchangeably in Medium B for detecting fluorescence of most fluorescent pseudo-